Differencial Serum Proteomes between Subphenotypes of Nonsyndromic Cleft Lip with or without Cleft Palate

Nonsyndromic cleft lip with or without cleft palate (nsCL/P)accounts for the majority of orofacial clefting in humans. New insights into the etiology of nsCL/P are requiring diversified and complementary investigative technologies. Since proteins act as the functional end products of gene expression, this investigation examined some distinct protein expression profiles of genes related to nsCL/P by analyzing differentially optimized peripheral serum protein mass spectrum peak values, utilizing Enhanced Laser Desorption Ionization Time of Flight (SELDI-TOF) technology. The differential peak values of protein 4119.1711 and 5639.1775 were discriminated repectively between cleft children and normal controls. 4079.4151, 5482.6131, 5684.2834, and 33310.9487 were cleft boys versus cleft girls. 2745.7079, 4473.4883, 6454.9339, 6560.7769, and 6654.3226 were cleft lip only versus cleft palate only. 4178.7872, 7842.6306, and 8147.0166 were cleft lip only versus cleft lip and palate. 4160.0878 and 5708.5676 were cleft lip only versus normal controls. These results indicate to be assistant for genomic etiology of nsCL/P.

Keywords: Proteomics; Genetics; SELDI-TOF-MS; Congenital abnormality; Nonsyndromic cleft lip with or without cleft palate (nsCL/P).